mycobacterium smegmatis urease test results

A. Organism is a coccus: go to Section B. The lactose tubes that were originally red due The rapid urease test confirmed a significantly lower rate of H. pylori infection in the recombinant Mycobacterium group (50%) than in the normal control (100%) and M. smegmatis (90%) groups (P < … Does not ferment mannose and rhamnose. production – Negative. culture revealed that it was a gram negative rod. Negative. Table 1 summarizes the radiometric urease test data. Enterotube – The tests and their results Citrate – Rapid Urease Test (RUT) The rapid urease test (RUT) is a popular diagnostic test for diagnosis of Helicobacter pylori. ALL GRAM NEGATIVE ORGANISMS Lactose – At room temperature, Serratia marcescens grew as bright, The usually red colonies had a bluish tinge when seen against the background of the blue DTC agar. the background of the blue DTC agar. Urease represents a critical virulence factor for some bacterial species through its alkalizing effect, which helps neutralize the acidic microenvironment of the pathogen. 643 0 obj <>/Filter/FlateDecode/ID[<2E3301542F7F9B44820E42E454E190F4>]/Index[630 24]/Info 629 0 R/Length 72/Prev 462517/Root 631 0 R/Size 654/Type/XRef/W[1 2 1]>>stream h�b```�t6�(Ad`f`B��f�%+.��q��3o���%;�X~����?��A~���s`����5m�����8:":,:8::8�A�� ������ b`{����X ,����S���~`,�N|�Y�U�-��������"���9H=*���xv��@��� J� TEST RESULTS. – Negative. They are gram -ve, catalase positive, oxidase negative, MR negative and VP positive bacteria. The rapid urease test confirmed a significantly lower rate of H. pylori infection in the recombinant Mycobacterium group (50%) than in the normal control (100%) and M. smegmatis (90%) groups (P < 0.05) (Fig. Urease. All isolates used in this investiga-tion gave standard urease test results which agreedwiththeliterature (3, 5). which results in the formation of acidic end products. The agar was a general enrichment agar The organism was able to grow in both aerobic and The colonies were pigmented only at room temperature or about 25 degrees Celsius. Fermentation tests. – Positive. the light pink background of the media. Does not ferment mannose and rhamnose. 653 0 obj <>stream and was not specific for the growth of Serratia an intermediate in the production of butyl glycerol in the fermentation of to the surface of the tube. Match. It was first reported in November 1884 by Lustgarten, who found a bacillus with the staining appearance of tubercle … M. smegmatis shares a number of morphological traits with M. tuberculosis including the distinctive waxy cell wall that provides a robust resistance to chemical disinfectants and sanitizers. Organism: Mycobacterium smegmatis . Biochemical Test of Mycobacterium tuberculosis. following Mycobacterium species gave positive results at 7 days: M. tuberculosis, M.kansasii, ... Asatisfactory urease test is needed to aid in ... semiquantitative catalase test results andposi-tive urease tests, so that the 93% of the M. Positive. Premium Questions. This ALL GRAM NEGATIVE ORGANISMS. ... Urease Test. the bacterial decarboxylation of lysine which Acid was produced causing a change in the color of the pH 2.2.3 Catalase test This is an antioxidan t enzyme responsible for eliminatin g molecules of hydrogen peroxide from the cells that are produced during respiration. shows a change in the pH from acidic to alkaline conditions. Biochemical Test of Mycobacterium tuberculosis. sodium salt as the sole source of carbon. endstream endobj startxref of ornithine, which results in the formation of an Phenylalanine deaminase – Negative. Gravity. The pH indicator therefore undergoes a color change (Ask instructor for results of this test if media is not available.) This indicted that the organism did not utilize DTC agar was the enrichment media we chose to grow Serratia marcescens. The test shows the bacterial decarboxylation A. Organism is a coccus: go to Section B. Organism: Mycobacterium smegmatis . The organism is capable of fermenting sorbitol The ammonia released results in the alkalinisation and an increase in the pH of the medium turning the indicator pink. Abstract. Negative. Antimicrobial susceptibility … The M. smegmatis ADHC was purified from M. smegmatis and the kinetic parameters of this enzyme … indicates that the organism does not have the enzyme tryptophanase The test can also be used to differentiate genera of gelatinase-producing bacteria such Serratia and Proteus from other members of the family Enterobacteriaceae. 1 B). Gram-positive Rod Nitrate - Catalase + Bacillus pumilis otilit + Catalase - Lactobacillus bulgaricus Nitrate + Catalase + Amylase - Amylase + Bacillus subtilis Mycobacterium smegmatis If visible, definitive feature is acid-fast cell wall Colonies tend to be white or cream in color, very dry If visible, definitive feature is endospore Also positive for fat hydrolysis Colonies tend to be … Adequately recording the procedures and results of each test (25 pts): • I (or anyone else) ... Mycobacterium phlei Sporosarcina ureae Staphylococcus aureus Staphylococcus epidermidis Ferments mannose and rhamnose Organism: Mycobacterium phlei. The gene encoding of an alcohol dehydrogenase C (ADHC) from Mycobacterium smegmatis was cloned and sequenced. DTC agar was the enrichment Created by. Mycobacterium smegmatis doesn't need so many copies of the genes because it doesn't require the high production of proteins when it is growing slow, while Escherichia coli does. are as follows: -. Voges- Proskauer – Negative. Indol formation – Negative. For strain 1, a slight decrease in turbidity is observed in the tube containing the bile salts (2nd from left), but the contents are almost as turbid as the control tube (far left); therefore, strain 1 … Serratia marcescens was able to The change in the color of the indicator from pale yellow to purple change in color several minutes after the reagent had been added. Growth On T- Soy agar. Motility stab. The presence was indicated by the development of a red color several Results of the bile solubility test are shown for two different strains of bacteria. to the presence of the pH indicator did not undergo a change in color and Sometimes the results of the tuberculin skin test are equivocal, particularly in persons who have been vaccinated with BCG or who live in areas where NTM are highly prevalent in the environment. The test detects the presence of aceylmethylcarbinol, a. Urease postive Organism: Klebsiella pneumoniae. 630 0 obj <> endobj The protein encoded by this gene has 78% identity with Mycobacterium tuberculosis and Mycobacterium bovis BCG ADHC. Optimal Growth Temperature: Determine the optimum growth temperature by growing the unknown at 25 o C and 37 o C and note where the organism grows best. The organism does not ferment adonitol H2S 5 % NaCl Tolerance = Negative (-ve), 68°C Catalase Test = Negative (-ve), Acid Fast Stain = Positive (-ve), Acid Phosphatase = Negative (-ve), Amidase Test = Positive (-ve), Arylsulphatase Test = Negative (-ve). NOTE: Methyl red differs from Phenol red (which is used in the fermentation test and the MSA plates) in that it is yellow at pH 6.2 and above and red at pH 4.4 and below. Mycobacterium tuberculosis: Is the pathogen responsible for tuberculosis. %%EOF Ferments mannose and rhamnose Organism: Mycobacterium phlei. products. endstream endobj 631 0 obj <>/Metadata 93 0 R/PageLayout/OneColumn/Pages 626 0 R/StructTreeRoot 119 0 R/Type/Catalog>> endobj 632 0 obj <>/Font<>>>/Rotate 0/StructParents 0/Type/Page>> endobj 633 0 obj <>stream H�|UMo�@��W�Ү����/8�9��D�oЃcpdl��=��wެq�4=`ƻ�=����";���:�"���"[?,嫲��z�+��`DV=�R0P�Be�\D|XʼWA"E��H�N;78���0. – Negative. Ornithine jill_uebele. |-S,��N*5E`��g��*ջ`��]�^(jW��5��?>�R�7+XxFݬ�s�r�\�X:�k)̞V ���w���uy�a��&?�}%�J��Nd����eN:6L�o�TA�y��sjxB5�2�;�R�%��|�\`��f�9_��2h�d:;���I9�~���Yϟ�׷)�ܕ���)�}�u�m0x�}D%ԟ�Ϝ�������%bkq�n_Dm����q-2,kPJ�C�s�ǂ0G>6��s�F&���(7���6�gw\�t���p ��Fi{*Rڏ�����5��l�(�w8�噖>�D&z����dq�}5N5�*fs��ءT�~�ǃ#x~��-��S��I���g�uc�]C�)�5��b;:�*v�wT`���7E�����衎�����1w�9CAࢨΊ���!��%|{\_�!���3�el� W.� ��›n�C���u͡�?��ckh��8|�)vm�2���ԛ���? Mycobacterium smegmatis urease test . Abstract. capable of producing pyruvic acid from the deamination of phenylalnine. Urea – b. Mycobacterium tuberculosis: Is the pathogen responsible for tuberculosis. that can hydrolyse urea to form ammonia and carbon dioxide. a. Urease … Principle: Many Mycobacterium species possess urease enzyme that hydrolyzes urea to form carbon dioxide and ammonia. endstream endobj 635 0 obj <>stream II. Mannitol Tubes. – Positive. Notes The quick growth rate of this microorganism is ideal for in-vitro testing, as other bacteria in this Genus may take several weeks to demonstrate growth. Test. Negative. Results of the bile solubility test are shown for two different strains of bacteria. Adequately recording the procedures and results of each test (25 pts): • I (or anyone else) ... Mycobacterium phlei Sporosarcina ureae Staphylococcus aureus Staphylococcus epidermidis Growth On DTC agar. Most of the possible unknown organisms will be mesophilic and will grow well at either temperature. The reaction results in the release of water and free oxygen (Palomino et al 2007). Two classes of catalase, thermolabile and thermostable, appear in … The most-commonly used phenotypic tests to identify and distinguish Mycobacterium strains and species from each other are described below.. Tests Acetamide as sole C and N sources. Gram stain of Serratia Mycobacterium smegmatis: Found on skin and mucous membranes, non-pathogenic : Acid-fast, non-sporing, Gram-positive rod. Mycobacterium smegmatis-Acid fast - Saprophyte (feeds on decaying matter)-gram positive rod. Guinea pig, died 22 days after inoculation with butter sample No 15 GP 60 ATCC 19420; GP 60 Originally Deposited as Mycobacterium butyricum Isolated by F Griffith, Ministry of Health, 1920.Redeposited by ATCC 1932 PRE:FR Ofthe 16 mycobacterial species in-cludedinthis study, 12(98isolates) arereported to beurease positive, and4 are urease negative (42 isolates). from pale yellow to purple. capable to reducing sulfur containing products. marcescens revealed that the organism was a gram negative bacteria, MacConkey's agar The motility stab showed that the organism was motile since it moved upward temperature or about 25 degrees Celsius. Mycobacterium smegmatis: Found on skin and mucous membranes, non-pathogenic : Acid-fast, non-sporing, Gram-positive rod. Arabinose Glucose – Since the gram stain of Serratia There are several methods for determining gelatinase production, all of which make use of gelatin as the substrate. 0 ... Urease Test. Lysine – Oxidation Negative. marcescens. Growth On DTC agar. The organism is not capable of fermenting lactose. The M. smegmatis ADHC was purified from M. smegmatis and the kinetic parameters of this enzyme showed that using NADPH as electron donor it … Procedure /Method of Gelatin hydrolysis test. Mycobacterium smegmatis is an acid-fast bacterial species in the phylum Actinobacteria and the genus Mycobacterium.It is 3.0 to 5.0 µm long with a bacillus shape and can be stained by Ziehl-Neelsen method and the auramine-rhodamine fluorescent method. (Ask instructor for results of this test if media is not available) Organism: Mycobacterium smegmatis II. �b��Ң���E c�w٧�ˋj[?Bw^?����o�U+r)}vY��R7OϭpNg?՝�L��jQ=m�6��z�^\���;�� �PRJrpO֫j�,��p�,뭸�����j�#ٮ�e������o�d�i7u��]�7�jAM_�`��٧�Z4竧E-dv���?D!��o/5u�`7�K��d��)>~�1e�2�ݟW��f��}iV�m߯�Ͷ�|�6�{Z����*�P�f7_�j1���ךb������y{�}!�OQ�1���,���B)�my��P��L�O�wc��R��b`�*^)�Y��t���:�Q(����"�(�Ǣ�v����ul��!�ו����sNc�ɛcL��:�40� Klebsiella pneumoniae Terms in this set (23) ... urease positive, grows on MSA, does not ferment mannitol. Biochemical tests were performed and the results showed a bacterium belonging to the group Mycobacterium smegmatis. H��T�N�@}�W��Z�7�]�"!$�J%$����aq��*�#{S�_ߙ�B�����3s沞g��U몵-\\L����S��|:o�kv�0�ϛ�C#�4J�`���ș�<���������./���L� ����'�,@A��(�g��`��)�M���d}�d���� [ �7w�����-n���*�T��d� ���>�t���HS�\,~\y~(��d�7�o�/H����$ ���=|�@� The colonies were pigmented only at room For strain 1, a slight decrease in turbidity is observed in the tube containing the bile salts (2nd from left), but the contents are almost as turbid as the control tube (far left); therefore, strain 1 is not S. pneumoniae. Media: KH 2 PO 4 (0.5 g), MgSO> 4 *7H 2 0 (0.5 g), purified agar (20 g), distilled water (1000 ml). The 14-day test identifies slower-growing species (M. marinum, M. xenopi) and some rapid-growers (M. smegmatis). It is also known as the CLO test (Campylobacter-like organism test). Nonmotile, acid-fast, non-sporing, Gram-positive rod. TEST RESULTS. Pathogenic mycobacteria express a functional urease, but its role during infection has yet to be characterized. If an organism which is red at 25 o C but only slightly pink at 37 o C, it is a mistake to presume the organism … Growth on Macconkey's agar. The organism does not have the enzyme urease anaerobic tubes. ted taxonomists to test directly the genetic relationships amongbacteria. Urease is an enzyme possessed by many Mycobacterium spp.
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